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CD35 抗体

This anti-CD35 antibody is a 小鼠 单克隆 antibody detecting CD35 in FACS, IHC, WB, IHC (p) 和 IHC (fro). Suitable for 人, Baboon, Cynomolgus 和 猕猴.
产品编号 ABIN337278
发货至: 中国

Quick Overview for CD35 抗体 (ABIN337278)

抗原

See all CD35 (CR1) 抗体
CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

适用

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人, Baboon, Cynomolgus, 猕猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD35 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

E11
  • 品牌

    IHC-plus™

    特异性

    Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.

    纯化方法

    Protein A purified

    免疫原

    Human acute monocytic leukaemia cells.

    Type of Immunogen: Cells

    亚型

    IgG1
  • 应用备注

    Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

    Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the diluted antibody to label 10^6 cells or 100 μL whole blood. Histology Positive Control Tissue: Tonsil.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4. Sourced in TCS.

    储存液

    Azide free

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

    别名

    CR1 / CD35

    背景

    Name/Gene ID: CR1
    Subfamily: Serine S1
    Family: Protease

    Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

    基因ID

    1378

    UniProt

    P17927

    途径

    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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